NireBryce

reality is the battlefield

the first line goes in Cohost embeds

๐Ÿฅ I am not embroiled in any legal battle
๐Ÿฆ other than battles that are legal ๐ŸŽฎ

I speak to the universe and it speaks back, in it's own way.

mastodon

email: contact at breadthcharge dot net

I live on the northeast coast of the US.

'non-functional programmer'. 'far left'.

conceptual midwife.

https://cohost.org/NireBryce/post/4929459-here-s-my-five-minut

If you can see the "show contact info" dropdown below, I follow you. If you want me to, ask and I'll think about it.


NoelBWrites
@NoelBWrites

I live in one of the largest cities in the US and I literally cannot find a place that will do a PCR covid test

Any testing sites left are doing only rapid tests, which... why would I go somewhere else (while sick!) to get a test if I can get the same unreliable results at home

SOME walgreens locations may still schedule a PCR for you, but it's $130 a pop and also I'd have to take public transportation to get there... while sick. With possible covid.

Fuck.


NireBryce
@NireBryce

this won't help you right now, but we demonstrably have the tools.

but there wasn't the support so i don't know what's needed to make it detect new strains, but maybe for PCR that's unnecessary.


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in reply to @NireBryce's post:

but there wasn't the support so i don't know what's needed to make it detect new strains, but maybe for PCR that's unnecessary.

the specifics depend on the protocol, but the process reports its result through a short DNA sequence ligated to a reporter chemical which reacts differently depending on whether a complementary DNA strand is bound to it or not, and there are modular systems for this. there's a small amount of work required to update it for new strains, but it's not insurmountable; probably the biggest undertaking would be verifying that the new test works.

my DIYbio is rusty but I've been thinking about building a lab in my basement for running unrelated preliminary tests instead of begging doctors to just fucking run them for three years each, so uh.

hmmm.

guess I need to put out an "ISO biochemistry dyke looking for a fun but tedious time" post on Lex.

That's the thing: if there's no support, if there's no access... we don't have the tools. I can't make my own covid test and I can find any that are affordable, so I don't have that particular tool.

Which is why the whole "we have the tools" messaging is so infuriating. Sure the tools may technically exist but most of us can't use them, so what good are them in a public health crisis.

Ideally we would build our own tools in a local, decentralized way so we don't depend on the state, but in the meantime, there's a public health emergency that the state insists we should be able to handle individually, and therefore pulled all support that would help us actually handle it

Idk I'm just so bitter about this

I know it's not helpful in the now (because it's a Project to get people together for it) and you're venting, so feel free not to read this, but on the topic of decentralized mutual aid:

running the tests is much simpler and cheaper than creating them.

their lab notebooks are online, as are their onboarding material and procedure documents, reagent lists and sources. they didn't have the 99$-500$ low volume open hardware thermocyclers we have available now, and you can make a two-vial Whirligig Centrifuge for like 4$ if a spinny boi is even needed (I've only skimmed).

someone would have to email them and some biologists to make sure it'll still pick up current strains, or learn how to modify it, but it's looking at more RNA than those mutations so might not even require tweaking.

it's just a time cost for the people involved.

it doesn't help you right now, because you can't build it in 14 days. but 10 people probably could in a week. They just need to be physically proximate and willing to donate time until the kinks in the process are worked out for the specific equipment.

I'm not sure how good of a use of time it is for a mutual aid thing because of how little people are pcr testing anymore as a population, but if people already have community networks it slots into them pretty easily. but everyone i know just forgot these existed, including myself until your post.

how to get people to form the groups required though, is the hard part. But we do have the tools, or at least ~trivial access to them with pooled money on a per-district basis. it's not perfectly decentralized, some of the reagents need to be bought from suppliers, but it's close.

(running our own bioreactors for the reagents is possible but requires much more capital outlay. DIYbio was a strong movement until about 2016 and then sorta faded from memory, but it still exists)

I can't dedicate time to this project for months if at all, but the procedure is much less involved than I thought for low test volume, and for higher test volume you can have more people running it in parallel on parallel hardware. I (and probably many others) forgot this existed until you brought it up. it feels like twenty years ago.

whether that's 130$ worth of time to the people involved is a separate question. but a lot of people within our various communities have time to give, who aren't given many other opportunities to use it (disabled people without enough stability or predictability of symptoms so they can't work jobs but can and want to do work (hi), etc)

I mean the whole thing is really cool! And organizing something like this also has the potential of helping in the future, pivoting to other needs, etc

I didn't know it was a thing so thank you for sharing. Which I now see I never thanked you in my first comment, sorry!

yeah honestly for this kind of thing the only thing that has worked for me to motivate people is to frame it as a "wouldn't it be fun/cool to try this out together?" sorta thing. But that's obviously only a one on one thing and like... has limited applications lol