they're in Henri-Georges Clouzot's harrowing (and first-rate) social-realist film The Wages of Fear, as two of the desperate men who take on the job of trucking nitroglycerin to the site of an oil-drilling blowout. that's Folco Lulli on the left, as Luigi; taller Yves Montand, on the right, as Mario. (Peter van Eyck, playing a character nicknamed 'Bimba', is off to the left in the background.)
Mario and Luigi aren't exactly uncommon names so I'm sure it's purely an accident that a brutal French film about the evils of capitalist "resource extraction" happens to line up with a couple of video-game characters. but it's still amusing.
~Chara