how feasible would it be to estimate capsaicinoids in hot-pepper samples with paper chromatography or thin-layer chromatography, and some kind of color-developing reagent to detect the vanillyl functionality in the capsaicinoids?
~Chara
- they/them
plural system in Seattle, WA (b. 1974)
lots of fictives from lots of media, some horses, some dragons, I dunno. the Pnictogen Wing is poorly mapped.
host: Mx. Kris Dreemurr (they/them)
chief messenger and usual front: Mx. Chara or Χαρά (they/them)
other members:
Mx. Frisk, historian (they/them)
Monophylos Fortikos, unicorn (he/him)
Kel the Purple, smol derg (xe/xem)
Pim the Dragon, Kel's sister (she/her)
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in reply to @pnictogen-wing's post:
To me, with a lab full of LC-MS systems, that's the obvious solution to every problem, but besides LC-UV and LC electrochemical detection, apparently a good bit of work on TLC methods and liquid-liquid extraction colorimetric methods has been done. Like:
https://www.sciencedirect.com/science/article/pii/S0039914016308293
https://www.sciencedirect.com/science/article/pii/S1021949816301776
Now, if you want something easier to do at home than methods using chloroform and toluene and n-hexane and so one, there might be more work to do!
thanks for the pointers! amusingly we were already wondering if capsaicinoids gave the indophenol reaction with N-chloroquinoneimines and, yep, it seems like they do! we were wondering about using that in conjunction with a paper / TLC chromatographic separation, but we do have a visible-light spectrophotometer in our hoard (that we desperately need to sort through and get into good order) so an extraction method followed by colorimetry might be a good approach.
the lack of good solvents available to the private citizen is...an issue. I am wondering if the commercially available 4-chlorobenzotrifluoride, which has been pushed into use in commercial solvents to replace things like methylene chloride, might serve the purpose.
~Chara